How to determine blood type through cross-matching?

Nov 09, 2023 Source: Cainiu Health
Dr. Liu Feng
Introduction
In general, cross-matching blood types involves observing whether agglutination occurs. If agglutination occurs, it indicates that the tested blood sample is incompatible with the known blood type sample, meaning the blood types are different. If no agglutination occurs, it indicates compatibility between the tested blood sample and the known blood type sample, meaning the blood types are the same.

Under normal circumstances, blood type is determined through cross-matching by observing whether agglutination occurs. The specific procedure is as follows:

First, prepare the blood sample to be tested and a blood sample with a known blood type. Place each sample into separate test tubes. Add equal amounts of antiserum to both tubes, selecting antiserum types that are opposite to the expected blood types of the samples being tested. Gently shake the tubes and observe whether agglutination occurs. If agglutination is observed, it indicates incompatibility between the tested sample and the known blood type sample, meaning their blood types differ. If no agglutination occurs, it indicates compatibility, meaning the blood types are the same.

It is recommended that patients have their blood type determined under medical supervision and seek prompt medical attention if any discomfort occurs, which helps support recovery.