What does a single-gene positive result in nucleic acid testing mean?
May 10, 2023
Source: Cainiu Health
Dr. Guo Xiheng
Introduction
A single-gene positive nucleic acid test result means that one of the two gene fragments targeted in the novel coronavirus nucleic acid detection test has tested positive. Specifically, the primary method for detecting novel coronavirus pneumonia is real-time fluorescent RT-PCR, which targets the open reading frame and nucleocapsid protein regions of the coronavirus genome.
In general, a single-gene positive nucleic acid test result means that one of the two targeted gene fragments in the SARS-CoV-2 nucleic acid test has returned positive. The detailed explanation is as follows:
The primary method for detecting SARS-CoV-2 infection is real-time fluorescent RT-PCR, which targets specific regions of the viral genome, such as the open reading frame (ORF) and nucleocapsid protein genes. Generally, laboratory confirmation of a positive case requires both target gene sequences to test positive in the same specimen. However, if only one target gene tests positive—referred to as single-gene positivity—it cannot be definitively concluded that the individual is infected with SARS-CoV-2. In such cases, re-sampling and retesting are required, along with antibody testing and imaging examinations for comprehensive evaluation. If infection is confirmed positive, immediate isolation and treatment are necessary. Medications such as ibuprofen sustained-release capsules and acetaminophen tablets may be taken under medical guidance. If the result is negative, individuals should continue protective measures and promptly report any abnormal symptoms.
During epidemic prevention and control periods, rooms should be regularly ventilated by opening windows, and visits to crowded places should be avoided. When going outdoors, wear a mask and avoid close contact with individuals exhibiting symptoms such as fever or cough.
