How to determine the therapeutic targets of targeted drugs
Jun 05, 2023
Source: Cainiu Health
Dr. Qi Zhirong
Introduction
Target identification for targeted therapies is typically achieved through methods such as gene sequencing and expression analysis, proteomic analysis, and bioinformatics analysis. Specifically, by performing genomic sequencing and expression analysis on tumor cells, genes associated with tumor initiation and progression can be identified, such as mutated oncogenes and aberrantly expressed signaling pathways.
To determine the therapeutic targets for targeted drugs, methods such as gene sequencing and expression analysis, proteomics analysis, and bioinformatics analysis are commonly used. The specific approaches are as follows:
1. Gene Sequencing and Expression Analysis
By sequencing and analyzing the expression profile of tumor cell genomes, genes associated with tumor development and progression can be identified, such as mutated oncogenes or abnormally expressed signaling pathways.
2. Proteomics Analysis
Using techniques such as mass spectrometry, changes in protein expression and post-translational modifications in tumor cells can be identified, helping to discover proteins involved in tumor initiation and progression.
3. Bioinformatics Analysis
By analyzing large-scale bioinformatics data—including gene microarrays, RNA sequencing, and protein-protein interaction networks—biological pathways and molecular mechanisms related to tumor development and progression can be revealed.
In addition to the above common methods, target identification can also be achieved through cell and animal model studies, clinical trials, and other approaches. However, the specific method should be determined based on individual patient conditions and diagnosed by a qualified physician.
