Are two lines on a pregnancy test stick definitely a sign of pregnancy?

A positive result on a pregnancy test strip (two lines) does not necessarily confirm pregnancy, although it does indicate a high probability of pregnancy. Pregnancy test strips work by detecting human chorionic gonadotropin (HCG) in urine, which reacts with specific reagents to produce two lines. However, the result may be influenced by multiple factors, including individual factors (such as medical conditions, elevated HCG levels following childbirth, or medication effects) and test-related factors (such as expired, damp, or contaminated test strips), all of which may lead to false-positive results. Therefore, when a pregnancy test strip shows two lines, women should not conclude pregnancy based solely on this result. Instead, they should consider other factors such as history of missed periods, sexual activity, and so on. If necessary, further medical examinations at a hospital are recommended, such as ultrasound scans, venous blood tests for HCG levels, and progesterone testing, to confirm whether pregnancy has occurred. Additionally, women should be aware of the possibility of false-positive results, and pay attention to the expiration date and storage conditions of the pregnancy test strips to ensure the accuracy of the test results.