Is it a problem if the red color on the pregnancy test stick has diffused?

Nov 15, 2025 Source: Cainiu Health
Dr. Zhang Lu
Introduction
If the red dye area on the pregnancy test strip is small, but the control line (C-line) is still clearly visible and the test line (T-line) is discernible (regardless of its intensity), and the reading is taken within the 5–10 minute window specified in the instructions, the result is generally reliable. The faint staining may be due to slightly excessive sample volume or minor moisture exposure affecting the test strip, but this does not impact result interpretation. You can determine pregnancy status based on the clear appearance of the lines.

Under normal circumstances, if the red smearing on a pregnancy test stick allows clear differentiation of the control line (C-line) and test line (T-line), and the result is read within the specified time, it is usually not a concern. However, if the lines are completely blurred and indistinguishable, or if the reading is taken beyond the recommended time, the result may be unreliable. If in doubt, it is advisable to seek medical advice promptly. Detailed explanations are as follows:

If the area of red smearing on the pregnancy test stick is small, and both the control line (C-line) and test line (T-line) remain clearly visible (regardless of the intensity of the T-line color), and the test is observed within the 5–10 minute window specified in the instructions, the result is generally reliable. The smearing may result from slightly excessive sample volume or minor moisture exposure affecting the test strip, but this does not interfere with interpretation. Pregnancy status can be determined based on the clearly visible lines.

If the test stick shows complete red smearing, making both the C-line and T-line indistinguishable, or if smearing appears only after exceeding the recommended reading time (more than 30 minutes, or 20 minutes for some products), the result may be invalid. In such cases, accuracy cannot be guaranteed. This may be due to user error (e.g., too much liquid applied), expired test strips, or moisture damage causing test failure. Pregnancy status cannot be reliably determined, and retesting is required.

Prior to using a pregnancy test, always check the expiration date and ensure the packaging is intact. Strictly follow the instructions regarding sample volume and reading time. If red smearing interferes with result interpretation, replace the test with a new one and retest using first-morning urine to ensure accurate results.

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