What is the chemiluminescence method for HIV testing?

Dec 03, 2025 Source: Cainiu Health

Dr. Liu Wenmin

Introduction

The chemiluminescence method for detecting HIV is a highly sensitive and specific immunoassay technique that determines infection status by detecting HIV antigens and antibodies in the blood. It is currently one of the commonly used clinical methods for screening and diagnosis. If there has been high-risk behavior or abnormal test results, it is recommended to seek medical attention promptly for further confirmation.

Chemiluminescence assay for HIV detection is a highly sensitive and specific immunoassay technique that determines infection status by detecting HIV antigens and antibodies in the blood. It is one of the commonly used clinical methods for screening and diagnosis. If there has been high-risk behavior or if test results are abnormal, it is recommended to seek medical attention promptly for further confirmation.

This method uses chemiluminescent signal amplification technology to convert the specific binding reaction between antigens and antibodies into measurable light signals. It can detect both the HIV p24 antigen and antibodies, thereby shortening the testing window period and enabling earlier identification of infection.

Compared with traditional enzyme-linked immunosorbent assays, the chemiluminescence method offers faster testing, higher accuracy, and effectively reduces false positive and false negative results. It is suitable for large-scale screening as well as individual precise testing, with a relatively simple procedure and minimal invasiveness for the examinee.

Prior to testing, avoid strenuous exercise and alcohol consumption; fasting is preferred. After the test, apply proper pressure to the blood draw site to stop bleeding, avoiding rubbing which may cause subcutaneous bruising. Keep the area clean and dry, remain calm while awaiting results, and follow scientific testing procedures to safeguard your health.

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How is HIV testing done?

In general, HIV refers to the human immunodeficiency virus. The human immunodeficiency virus can be detected through rapid antibody testing, enzyme-linked immunosorbent assay (ELISA), antigen-antibody combination testing, nucleic acid testing, Western blot testing, and other methods. If experiencing any discomfort, timely medical consultation is recommended. Different testing methods have their own advantages, limitations, and appropriate use scenarios, and the choice of test should be based on specific circumstances.

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What is the normal value for combined HIV antigen and antibody testing?

The normal reference value for combined HIV antigen and antibody testing is a result of less than 1.0 S/CO, indicating that HIV antigen or antibody was not detected in the sample, which is considered a negative result. A value greater than or equal to 1.0 S/CO is considered positive or in the gray zone, requiring further confirmation. If there has been high-risk behavior or abnormal test results,

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What are the symptoms of HIV infection?

After HIV infection, symptoms such as persistent low-grade fever, night sweats, painless lymphadenopathy, trunk maculopapular rash, and oral mucosal leukoplakia may occur, typically appearing 2–4 weeks after infection. In later stages, multiple organ systems may be affected. If there is a history of high-risk exposure and any of the above symptoms develop, prompt HIV testing and medical evaluation are recommended.

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Which generation of HIV test

Under normal circumstances, current HIV testing is primarily based on third- and fourth-generation tests, with fifth-generation testing emerging as a newer technology. Different generations vary in terms of detection targets and window periods, so selection can be made according to individual needs. After high-risk exposure, it is recommended to prioritize fourth-generation antigen/antibody combination tests or nucleic acid testing (NAT) for early screening, followed by retesting after the window period for confirmation. Testing should be conducted at正规 medical institutions, taking into account both the timing of the test and personal circumstances.

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Will HIV be tested during blood draw for prenatal checkups?

Prenatal blood tests include screening for several infectious diseases, one of which is HIV testing. By detecting HIV antibodies or viral nucleic acids in the blood, it can be determined whether a pregnant woman is infected. Since the HIV virus may be transmitted from mother to child through vertical transmission, early detection allows timely interventions such as antiviral treatment to reduce the risk of fetal infection and ensure the safety of both mother and child.

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What tests are used to detect HIV/AIDS?

HIV testing requires selecting appropriate methods based on the stage of infection and specific needs. Commonly used testing methods include HIV antibody screening tests, HIV antibody confirmatory tests, HIV nucleic acid testing, viral load testing, and rapid test kits. The choice of testing method should take into account the time since exposure and the specific situation, and testing should be performed as early as possible after high-risk behavior.

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How long after infection can HIV be detected?

HIV can usually be detected by nucleic acid testing 2–4 weeks after high-risk behavior. The window period for antibody testing is approximately 3–12 weeks, with most people obtaining preliminary test results within 6 weeks. A final diagnosis should be confirmed based on follow-up test results. If symptoms such as persistent fever or rash occur after high-risk behavior, or if there are concerns about test results, it is recommended to seek medical advice promptly.

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Does taking PEP medication affect HIV testing?

In general, whether taking HIV post-exposure prophylaxis (PEP) affects HIV testing depends on the type of test used. PEP may affect antibody tests, potentially delaying detection, but has minimal impact on nucleic acid testing, which can usually be performed normally. During PEP treatment, it is essential to strictly follow medical instructions and not discontinue or reduce medication without guidance. Keep a record of medication times and any bodily reactions, and choose the appropriate testing method and timing as advised by your doctor.

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