How is immunohistochemical testing performed?
Nov 20, 2023
Source: Cainiu Health
Dr. Lu Haiying
Introduction
Immunohistochemistry is a pathological examination technique based on chemical methods and is widely used in tumor diagnosis. If this test is needed, it is recommended to visit a正规 hospital and undergo the procedure under the guidance of a qualified physician to ensure the accuracy of the results. If abnormal findings are detected, prompt consultation with a doctor is advised to obtain professional advice.
Generally, immunohistochemistry is a pathological examination technique based on chemical methods and is widely used in tumor diagnosis. The specific procedure is as follows:
1. Obtain tissue samples through biopsy or surgery, ensuring sufficient tissue is collected for analysis.
2. Fix the tissue samples using substances such as formaldehyde or ethanol to preserve the integrity of cellular structures.
3. Section the fixed tissue samples into slices, typically 3 to 5 micrometers thick.
4. Select the target proteins for detection and label them using specific antibodies.
5. Apply the selected antibodies to the tissue sections, allowing them to bind to specific cells within the tissue via fluorescent staining.
6. Examine the stained tissue sections under a microscope and document the findings.
If this test is required, it is recommended to visit a正规 hospital and undergo the procedure under the guidance of a qualified physician to ensure accurate results. If any abnormalities are detected, prompt consultation with a doctor is advised to obtain professional advice.
