Is it possible for nucleic acid tests to produce false-positive results?
Dec 03, 2024
Source: Cainiu Health
Dr. Yang Ziqi
Introduction
Under normal circumstances, nucleic acid testing is a molecular biology technique used to detect specific viral nucleic acid sequences. Whether false positives may occur depends on the specific situation. If you feel unwell, it is recommended to seek medical attention promptly. Choose a qualified healthcare facility for nucleic acid testing to ensure the standardization and accuracy of sample collection, transportation, processing, and testing.
Generally, nucleic acid testing, as a molecular biology technique, is used to detect specific viral nucleic acid sequences to determine whether an individual is infected with a virus. Whether false positives may occur depends on specific circumstances. When operations are performed in accordance with established protocols, false positives generally do not occur; however, sample contamination may lead to false positive results. If you experience any discomfort, timely medical consultation is recommended. Detailed analysis is as follows:
Nucleic acid testing itself has very high specificity and sensitivity, enabling accurate identification and amplification of target nucleic acid sequences without reacting to non-target sequences. Therefore, false positive results generally do not occur.
However, if the sample becomes contaminated during collection, transportation, or processing—for instance, cross-contamination with other positive samples—this may lead to false positive results. Additionally, issues with the quality of the test kit, such as improper probe or primer design, may also lead to non-specific amplification and thus produce false positive results.
It is recommended to undergo nucleic acid testing at正规 medical institutions to ensure standardization and accuracy in sample collection, transportation, processing, and testing.
