When is the most accurate time to test the six hormone levels?
Jul 23, 2025
Source: Cainiu Health
Dr. Yang Ziqi
Introduction
The "six sex hormone tests" refer to the six-item examination of sex hormones. There is no definitive timing considered most accurate for conducting these tests. The appropriate time for testing may vary depending on the specific purpose of the examination. Before the test, an 8- to 12-hour fast is required. Additionally, sufficient sleep should be ensured the night before, and strenuous exercise, emotional stress, and sexual stimulation should be avoided, as these factors may affect the secretion of hormones such as prolactin.
Generally speaking, the "six sex hormone tests" refer to the six-item sex hormone panel. There is no specific time considered most accurate for conducting these tests. The timing of the six sex hormone tests varies depending on the purpose of the examination. Detailed analysis is as follows:
If assessing basic ovarian function is the goal, an optimal time for testing is on day 2 to day 5 of the menstrual cycle, which is the early follicular phase. Hormone levels during this stage are relatively stable and the measured values can accurately reflect ovarian reserve capacity—for example, values such as follicle-stimulating hormone (FSH) and luteinizing hormone (LH) can help determine whether ovarian function is normal. If the menstrual cycle is irregular or there is prolonged amenorrhea and prompt results are needed, testing can be performed at any time, although interpretation of the results should be combined with clinical circumstances for comprehensive analysis.
If monitoring ovulation is the purpose, testing can be done around the expected ovulation date, generally on day 12 to day 14 of the menstrual cycle. The focus is primarily on changes in hormones such as luteinizing hormone (LH) and estradiol (E2), which will show a peak prior to ovulation and can assist in determining the timing of ovulation. To evaluate luteal function, progesterone levels are typically tested on day 21 to day 23 of the menstrual cycle or seven days after ovulation. Low progesterone levels may suggest inadequate luteal function.
Fasting for 8 to 12 hours before the test is required. Ensure adequate sleep the night before, and avoid strenuous activity, emotional stress, and sexual stimulation, as these factors may affect the secretion of hormones such as prolactin. If taking sex hormone medications, inform your doctor in advance as they may interfere with test results.
