How to use an HIV test kit

Jan 25, 2025 Source: Cainiu Health
Dr. Liu Feng
Introduction
Under normal circumstances, the use of HIV test strips includes preparation, cleaning and disinfection, sample collection, waiting for the reaction, and reading the results. If necessary, it is recommended to consult a doctor. Before using HIV test strips, ensure that the strips are within their expiration date and that the packaging is intact and undamaged to guarantee the test performance and accuracy.

Generally, the use of HIV test strips involves preparation, cleaning and disinfection, sample collection, waiting for reaction, and reading the result. If necessary, it is recommended to consult a doctor. The detailed steps are as follows:

1. Preparation

Before using the HIV test strip, ensure that the test strip is within its expiration date and that the packaging is intact and undamaged to guarantee the test performance and accuracy. Prepare auxiliary supplies such as a lancet, alcohol swabs, and disposable gloves.

2. Cleaning and Disinfection

Use an alcohol swab to clean and disinfect the blood collection site or oral cavity to reduce the risk of infection, ensure hygiene during the testing process, and thereby enhance the accuracy of the test.

3. Sample Collection

For blood testing strips, gently prick the collection site with a lancet and squeeze out an appropriate amount of blood. For oral fluid testing strips, place the oral swab on the gum area and rub back and forth for 5-6 seconds. Shake the diluent in the tube well, then immerse the oral swab into the diluent, stirring it back and forth 5-10 times.

4. Waiting for Reaction

Wait for the reaction time specified in the instructions. During this period, avoid touching or moving the test strip, as this may affect the result. The reaction time may vary depending on the type of test strip, generally between 15-30 minutes.

5. Reading the Result

Read the result within the specified time. If two lines appear on the test strip, it usually indicates a positive result, meaning the person may be infected with HIV. If only one red line appears, it typically indicates a negative result, meaning no HIV infection was detected.

However, the result of an HIV test strip cannot serve as the sole criterion for confirming an HIV diagnosis. If the result is positive, it is essential to visit a hospital promptly for further testing.

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